The Functional Genomics Laboratory is equipped with an Illumina iScan for SNP genotyping and expression arrays. Illumina arrays are based on oligo coated beads each unique to a specific SNP locus. The beads are randomly distributed and located in 3 micron wells etched in a slide. SNPs are detected in two formats
- Goldengate - using Sentrix BeadChips
- Infinium Bead Arrays - High density Beadchips with multiple formats up to 5 million SNPs.
Both technologies include predesigned or custom assays.
For a full list of available products or an overview of the beadarray technology see the Illumina website at
http://www.illumina.com/.
Custom SNP design for Goldengate assays is also available through Illumina.
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Hybridized Goldengate 96 sample matrices or high
density Infinium slides are scanned in the Illumina Bead Scanner
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Goldengate image of a single bead cluster showing 1536 genotypes for one DNA sample.
SNP genotypes: AA (green), BB (red), AB (yellow) |
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Analysis of bead array signals is carried out using the Illumina Beadstudio software.
Genotype calls are exported in tabular format compatible for linkage or association studies with third party programs.
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SNP cluster showing genotype calls for 96 DNA samples.
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For additional information and specific project pricing contact Dr. Mark Band (markband@illinois.edu)217-244-3930.
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