The Taqman ABI 7900 real time PCR machine is operational and available for use by all university researchers. The use of low volume reactions and 384 well plates conserves both reagents and budget. Taqman Low Density Arrays (TLDA) can also be run on the 7900.  In addition, our machine is equipped with an autoloading robot allowing 24 hour use of the machine. Real time PCR is one of the most sensitive and accurate assays for RNA expression analysis allele discrimination or DNA quantification. At present all users are trained for sample setup and analysis by Functional Genomics Lab staff. Users prepare their samples independently and bring in their PCR plates ready to run. The Primer Express and Sequence Analysis software is available to be used in the Functional Genomics Lab.

For University of Illinois researchers there is a $35.00 fee/plate. This covers only maintenance and service contracts. Please provide an account number when making an appointment to use the machine. Plasticware and reagents required for Real-Time PCR analysis.

We strongly suggest reading the following technical bulletins published by Applied Biosystems explaining the principles of Real-Time PCR before planning your assays.

Real Time PCR User Instructions
Designing MGB Probes and Primers
Essentials of realtime PCR
Guide to relative quantitation
Rapid Assay developement guidelines
Real time vs. traditional PCR
User bulletin 2

For more information contact Dr. Mark Band at the Functional Genomics Lab. Tel: (217)244-3930
E-mail: markband@illinois.edu

Functional Genomics Unit
Mark Band, Ph.D - Director
356 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-3930     FAX: (217) 265-5066      Email: markband@illinois.edu