Flow Cytometry Facility - Immunophenotyping

Immunophenotyping

DIRECT STAINING
Fluorescent probe attached to primary antibody

Protocols for Direct Immunofluorescence Staining with Flow Cytometry

INDIRECT STAINING
Fluorescent probe attached to a secondary antibody

Protocols for Indirect Immunofluorescence Staining with Flow Cytometry

AVIDIN-BIOTIN METHOD
Biotynylated antibody can bind many avidins (streptavidins) - fluorochromes complex to improve sensitivity of assay

Zenon® labeling technology

Zenon technology is based on the complexation of primary antibodies with dye– or enzyme–labeled Fab fragments of secondary antibodies directed against their Fc regions.

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Antibodies-Avidins-Lectins-and-Related-Products/Zenon-Technology-Versatile-Reagents-for-Immunolabeling.html

General Staining Techniques

Vendors

Flow Cytometry Facility
Barbara Pilas, Ph.D - Director
231 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-0559 FAX: (217) 244-0446 Email: http://illinois.edu/

Last edited: 14 May 2007