Immnological Resource Center
Service fees
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The fees presented here are for the academic year of 2010-2011, starting on July 1, 2010 and for
University of Illinois users only. Non-UI clients will be served for an additional overhead fee.
Please see fee and other requirement information for off-campus clients at the end of the webpage.
Contact us for any questions at irc@life.illinois.edu or call at 217-244-0558.
A. Polyclonal Antibody Generation
Animals (species)
|
Antigen quantity required |
*Antigen purity required
|
Testing sample
|
Final product
|
Time (weeks) |
Fees
|
Rabbit
(NZW) |
1.5mg for one animal |
>90% |
2 x 10ml serum from each animal |
70ml serum from each animal |
2-5 months |
$635 (use 1 animal)
|
Chicken
(farm chicken) |
1.5mg for one animal |
>90% |
500ul serum from each animal
|
10 eggs |
3-4 months |
$240 (use 1 animal)
|
*Less than 90% purity is acceptable under certain conditions.
B. New Mouse Monoclonal Antibody Production
| Animal |
Antigen quantity required |
Antigen purity required |
Time frame |
Products |
Fees |
| Balb/c mice (5) |
1.5mg |
>80% |
4-6 months |
Hybridoma cell lines and medium |
$2,320 |
C. Monoclonal Antibody Production with Hybridoma Cells
Methods |
Products |
Antibody concentration |
Time frame |
Fees |
Conventional flaks culture |
hybridoma medium
|
0.02 -0.1mg/ml |
7-20 days |
$1/ml |
Integra CL350 bioreactor |
36ml
hybridoma medium |
~1mg/ml |
9 weeks |
$420
|
Integra CL100
Bioreactor |
90ml
hybridoma medium |
~1mg/ml
|
9 weeks |
$600 |
Mouse ascites |
Crude ascites fluid |
20ml
1-6mg/ml
|
9 weeks |
$450 |
Notes:
Hybridoma medium products contain a small quantity of cow antibody molecules and other serum proteins
introduced by fetal bovine serum. Serum-free medium production is available at request and for different fees
D. Antibody Purification
Service |
Starting material |
Fee |
Time |
IgG purification with Protein A |
Rabbit serum |
$525 for 20mg purified antibody |
1-3 weeks |
IgG purification with Protein G |
Hybridoma medium |
$220 from 100 ml hybridoma medium (2-5mg) |
Frozen cells( 2 Special package) |
$350 for 5mg purified antiboby |
Mouse ascites |
$570 for 20ml ascites |
Antigen affinity purification |
Serum/ascites |
Ask for quote
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1 We offer significant discounts for protein G/A purification with used(1-2times) protein G/A columns. Contact us for quote at irc@life.illinois.edu.
2 Guarantee 5 mg protein G purified monoclonal antibody produced from a frozen hybridoma cell line generated by IRC.
E. Miscellaneous Antibody Services
Service |
Fee |
Quantity |
ELISA |
$45 |
One 96-well plate |
Antibody isotyping |
$40 |
One cell line
($15 for Additional Cell line) |
Peptide conjugation to KLH |
$75 |
One peptide to one carrier protein (KLH, BSA or OVA) |
Antibody biotinylation |
$50 |
1-10mg antibody |
Antibody biotinylation |
$75 |
*<0.5 mg antibody |
F. Biacore 3000 Services
Service |
Fee
|
|
Instrument individual training
|
$160 (~6 hours)
|
Instrument hourly rate (user operation)
| $40
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For Biacore training, consultation and services, please contact lab supervisor Rachel Breitenfeld at 217-244-0558/ rbtnfld@illinois.edu or Brain Imai at 217-244-9443/bsimai@illinois.edu. For Biacore technical information and products, please visit http://www.biacore.com.
Facility users other than State of Illinois institutions are subject to a 25.3.% indirect cost rate for federally funded projects. For non-federally funded and commercial projects the indirect cost rate is 30%. For off-campus projects exceeding $5,000 a contract must be negotiated through University of Illinois Grants and Contracts. For further information contact Dr. Mark A. Mikel (217.244.0144, mmikel@uiuc.edu), Associate Director of the Biotechnology Center.
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